of the light signal scattered at a small angle in the forward direction. : FSC-A is primarily used to estimate
To understand FSC-A, one must first understand what the "FSC" part means. detects light that passes through a cell and continues in a forward direction (typically 0.5° to 15° off the axis of the laser beam). Unlike Side Scatter (SSC), which detects refracted and reflected light at 90°, FSC intensity is directly proportional to the cell's surface area or diameter. of the light signal scattered at a small
: The intensity of the FSC signal is generally proportional to the diameter of the cell. Larger cells, such as monocytes, produce higher FSC signals than smaller cells, like lymphocytes. such as monocytes